Quantitative Analysis of Phellodendron Bark Based on Near Infrared Spectroscopy

SU Xiu-hong; XIE Hui-ying; WANG Feng-qing; LEI Jing-wei; WANG Li-na; XIE Cai-xia

doi:10.13422/j.cnki.syfjx.2016080087

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Quantitative Analysis of Phellodendron Bark Based on Near Infrared Spectroscopy

更新时间：2024-01-04

- Quantitative Analysis of Phellodendron Bark Based on Near Infrared Spectroscopy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 8, Pages: 87-91(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016080087
  CLC： R284.1
- Published：2016
- 稿件说明：
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SU Xiu-hong, XIE Hui-ying, WANG Feng-qing, et al. Quantitative Analysis of Phellodendron Bark Based on Near Infrared Spectroscopy[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(8): 87-91.
DOI：

SU Xiu-hong, XIE Hui-ying, WANG Feng-qing, et al. Quantitative Analysis of Phellodendron Bark Based on Near Infrared Spectroscopy[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(8): 87-91. DOI： 10.13422/j.cnki.syfjx.2016080087.

摘要

目的: 建立一种能够同时快速测定黄柏药材中小檗碱和黄柏碱含量的方法。方法: 以HPLC所测样品中小檗碱和黄柏碱的含量为参考值

利用近红外光谱分析技术

将参考值与样品的近红外光谱进行关联

采用偏最小二乘法(PLS)建立黄柏中小檗碱和黄柏碱的定量分析模型。结果: 所建立的小檗碱和黄柏碱的定量分析模型的内部交叉验证决定系数(R2)分别为0.99713

0.98923

校正均方差(RMSEC)分别为0.0823

0.0215

验证均方差(RMSEP)分别为0.0717

0.0166

性能指数PI分别为95.6

93.5。结论: 利用近红外光谱技术建立的定量分析模型

能够对黄柏药材中小檗碱和黄柏碱的含量进行无损快速测定。

Abstract

Objective: To establish a method for simultaneous rapid determination of berberine content and phellodendrine content in Phellodendron Bark. Method: With the berberine content and phellodendrine content determined in high performance liquid chromatography (HPLC) as the reference values

near infrared spectra of the samples were associated with the reference values above

and then the quantitative analysis models of berberine and phellodendrine were established by using partial least squares (PLS) method. Result: The correlation coefficient (R2) was 0.99713 and 0.98923 respectively for quantitative analysis model of berberine and phellodendrine. The root mean square error of calibration (RMSEC) was 0.0823 and 0.0215 respectively. The root mean square error of predication (RMSEP) was 0.0717 and 0.0166 respectively. The performance index (PI) was 95.6 and 93.5 respectively. Conclusion: The quantitative analysis model established by using near-infrared spectroscopy is rapid and nondestructive to determine the content of berberine and phellodendrine in Phellodendron Bark.

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Related Author

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ZHANG Di-wen

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XIE Cai-xia

HU Xiao-li

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Related Institution

Institute of Basic Medical Sciences of Xiyuan Hospital，China Academy of Chinese Medical Sciences

Graduate School of China Academy of Chinese Medical Sciences

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Institute of Information Traditional Chinese Medicine（TCM）， China Academy of Chinese Medical Sciences

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