Effect of Notoginseng Radix et Rhizoma Saponins,Salidroside and Effective Components of Astragali Radix in Mobilizing Bone Marrow Mesenchymal Stem Cells in Rat Myocardial Infarction Model

ZHANG Bao-xia; ZHANG Jin-sheng; ZHANG Yang-yang; ZHU Hui-fang

doi:10.13422/j.cnki.syfjx.2016080137

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Effect of Notoginseng Radix et Rhizoma Saponins,Salidroside and Effective Components of Astragali Radix in Mobilizing Bone Marrow Mesenchymal Stem Cells in Rat Myocardial Infarction Model

更新时间：2024-01-04

- Effect of Notoginseng Radix et Rhizoma Saponins,Salidroside and Effective Components of Astragali Radix in Mobilizing Bone Marrow Mesenchymal Stem Cells in Rat Myocardial Infarction Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 8, Pages: 137-142(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016080137
  CLC： R-332;R285.5
- Published：2016
- 稿件说明：
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ZHANG Bao-xia, ZHANG Jin-sheng, ZHANG Yang-yang, et al. Effect of Notoginseng Radix et Rhizoma Saponins,Salidroside and Effective Components of Astragali Radix in Mobilizing Bone Marrow Mesenchymal Stem Cells in Rat Myocardial Infarction Model[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(8): 137-142.
DOI：

ZHANG Bao-xia, ZHANG Jin-sheng, ZHANG Yang-yang, et al. Effect of Notoginseng Radix et Rhizoma Saponins,Salidroside and Effective Components of Astragali Radix in Mobilizing Bone Marrow Mesenchymal Stem Cells in Rat Myocardial Infarction Model[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(8): 137-142. DOI： 10.13422/j.cnki.syfjx.2016080137.

摘要

目的: 探讨三七总皂苷、红景天苷、黄芪有效组分对心肌梗死大鼠模型骨髓间充质干细胞(bone mesenchymal stem cells

BMSCs)动员作用。方法: 选取SPF级SD大鼠

参考Olivette方法建立急性心肌梗死模型

造模成功大鼠随机分为活血组、益气组、益气活血组、模型组和正常组

各组再随机按1

28 d时间点再进行分组。活血组8 mg·kg-1血塞通软胶囊、益气组5 mg·kg-1黄芪颗粒、益气活血组7 mg·kg-1诺迪康胶囊

模型组予生理盐水ig

正常组不给药

正常组和模型组以等量生理盐水ig

1次/d

共28 d。流式细胞仪检测不同时间点血液和骨髓中CD54

CD106

CD105

CD117阳性细胞百分数

酶联免疫吸附测定(ELISA)法检测不同时间点血液和骨髓中干细胞因子(SCF)变化。结果: 模型组外周血和骨髓中CD105

CD117

SCF从1 d开始升高

14 d到高峰

其后外周血和骨髓中CD105

CD117

SCF含量降低;活血组、益气活血组、益气组外周血和骨髓中CD105

CD117

SCF含量上升优于模型组(P<0.05)。活血组CD105

CD117

SCF上升优于益气活血组、益气组。模型组外周血和骨髓中CD54

CD106在1 d到高峰

其后逐渐降低

活血组外周血中的CD54

CD106降低明显较模型组相比(P<0.05)。益气组与模型组相比变化不明显

但活血化瘀方药降低CD54

CD106最明显。结论: 三七总皂苷、红景天疳、黄芪有效组分均有动员骨髓间充质干细胞作用

但活血化瘀方药动员效果明显。

Abstract

Objective: To investigate the effect of Notoginseng Radix et Rhizoma saponins

salidroside and effective components of Astragali Radix in mobilizing bone marrow mesenchymal stem cells in the rat model of myocardial infarction. Method: The SD rat model of myocardial infarction was established by Olivette method. The successfully modeled rats were randomly divided into:Huoxue group

Yiqi group

Yiqi and Huoxue group

model group and normal group

and then subdivided according to time points at 1

28 d. Huoxue group was given 8 mg·kg-1 of Xuesaitong soft capsule. Yiqi group were treated with 5 mg·kg-1 of Huangqi granule. Yiqi and Huoxue group was given 7 mg·kg-1 of Nuodikang capsule. Model group was given normal saline. Normal group received no drug. Normal group and model group were administered with equivalent normal saline by gavage once a day for 28 days. The positive cells percentage of CD54

CD106

CD105

CD117 in peripheral blood and bone marrow were detected by flow cytometry at different time points

and ELISA method was used to detect the expression of stem cell factor (SCF) in peripheral blood and bone marrow at different time points. Result: SCF level and positive cells percentage of CD105

CD117 content increased in model group since the first day

reached the peak on the 14th day

and then decreased gradually. But the increasing trend was superior in Huoxue group

Yiqi group

Yiqi and Huoxue group (P<0.05)

particularly Huoxue group was the best. The prescription of promoting blood circulation to remove blood stasis showed an obvious mobilizing effect. Positive cells percentage of CD54

CD106 in peripheral blood and bone marrow in model group reached the peak on the first day

and then decreased gradually

but Huoxue group showed a superior decreasing trend in CD54

CD106 than model group in peripheral blood (P<0.05). Compared with model group

CD54 and CD106 in bone marrow had no significant change. Conclusion: Notoginseng Radix et Rhizoma saponins

salidroside and effective components of Astragali Radix can mobilize bone marrow mesenchymal stem cells

and the mobilizing effect of the prescription of promoting blood circulation to remove blood stasis is the most obvious.

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